FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6008050
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA1109891.8473381546425212TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT347300.5780577724885778TruSeq Adapter, Index 20 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT195600.037.27318252-53
TGCCGTC198000.036.7641750-51
TATGCCG201800.036.41318548-49
CGTATGC213850.034.4280146-47
CTTGAAA211400.034.07543662-63
TGCTTGA215050.033.6296160-61
CTCGTAT225550.032.31395344-45
GTCTTCT241650.030.35090854-55
ATCTCGT238200.030.31706842-43
TCTGCTT247450.030.11942558-59
TGAAAAA252900.028.51218664-65
GCCGTCT194150.028.34102852-53
CTTCTGC264000.028.21326456-57
CGTCTTC197200.027.9182254-55
ATGCCGT200650.027.43682150-51
TTATCTC282350.026.03908240-41
CTGCTTG216900.025.73251760-61
GTATGCC219350.025.39011648-49
CCTTATC291600.025.28637738-39
TCGTATG220000.025.26112646-47
Produced by FastQC (version 0.11.5)