FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6008050
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC946611.5755694443288588Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA152100.034.73538250-51
CCGTATC158700.033.9707948-49
GCCGTAT159900.033.20099348-49
CGCCGTA164450.032.60700246-47
GTATCAT167700.032.05122850-51
ATCATTA181100.031.65264752-53
TCGCCGT160400.031.01101346-47
GTCGCCG172800.030.46801244-45
CATTAAA194300.030.18567554-55
TCATTAA183650.030.06510254-55
TATCATT179800.029.48646552-53
GGTCGCC181150.028.59630444-45
TGGTCGC178750.027.97931542-43
GTGGTCG189600.026.13014642-43
TCGGTGG230950.023.70620238-39
CGGTGGT211450.023.61441840-41
ATTAAAA241650.023.31268356-57
CGCCGGA10400.023.29237746-47
GGTGGTC219500.023.0268440-41
GGCGCCG21750.022.27110344-45
Produced by FastQC (version 0.11.5)