FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4561838
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC60140.13183282703156052Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA1953.5104858E-715.24211990-91
GTCGCGT1959.0002824E-512.4697288-89
ATCGGAC2451.262633E-410.26583186-87
CGTATCA35700.010.0814350-51
CGCCGGA4952.6375346E-109.50202646-47
CGTCGCG2852.817308E-49.47990588-89
CCGTATC39050.09.33550748-49
TCGGACG2400.00701277569.0059188-89
TAGCTCG3351.442037E-48.87227890-91
CGCGTAG2750.0021419268.84294890-91
GCCGTAT40650.08.79448248-49
GATCCGA4952.4208202E-78.73300488-89
TGGTCGC61650.08.58799594-95
GCGCGTC2850.0029599798.53299392-93
GTATCAT45900.08.45610150-51
CGCCGTA42900.08.27772646-47
GATCGGG8007.73086E-68.2016711
GGTCGCC58050.08.14772194-95
CGAGCGT3050.00545603137.973452692-93
TCATTAA52450.07.85299454-55
Produced by FastQC (version 0.11.5)