FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25465733
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC433510.17023268091281724TruSeq Adapter, Index 9 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT215100.014.53184350-51
TATGCCG220500.014.05743546-47
TGCCGTC225650.013.70497148-49
GCCGTCT209900.012.55900848-49
CGTATGC251350.012.32849644-45
CGTCTTC232700.011.512320550-51
ATGCCGT227600.011.47790146-47
CTCGTAT282150.010.73862642-43
CTTGAAA319100.010.07580560-61
TCGTATG270700.09.70163542-43
GTATGCC277900.09.55282344-45
TGCTTGA342900.09.424958-59
ATCTCGT332950.09.04308540-41
CTGCTTG314850.08.94470256-57
TCTCGTA305700.08.30345140-41
GTCTTCT429200.07.84358652-53
TCTGCTT459150.07.46271156-57
GCTTGAA380150.07.43948658-59
CTTCTGC493050.06.94476454-55
TGAAAAA512200.06.726838662-63
Produced by FastQC (version 0.11.5)