FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9956754
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29650.022.55943146-47
CGTATGC32400.020.76483744-45
CTCGTAT33600.019.36172942-43
ATCTCGT43350.015.11655440-41
CCGTCTT46000.015.00675150-51
TGCCGTC46150.014.5969248-49
TCGTATG32900.011.77819744-45
ATGCCGT36800.09.89669348-49
TCTCGTA37950.09.38452442-43
GAATCTC81850.08.81846838-39
GCCGTCT45350.08.39825750-51
TGCTTGA88200.08.18458658-59
AATCTCG45000.08.07256940-41
GTATGCC49400.08.04703746-47
TTGAATC88850.07.990317336-37
CTTGAAA99050.07.26399460-61
ACACTTG107800.07.00499332-33
CGTCTTC57950.06.9243752-53
ACTTGAA124600.06.231412434-35
GTCTTCT136450.05.72491952-53
Produced by FastQC (version 0.11.5)