FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4500285
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16150.015.03862246-47
CGTATGC18450.013.14477444-45
CCGTCTT21300.012.408627550-51
TCGATTA2900.00457716811.4618325
TGCCGTC22950.010.89399148-49
CTCGTAT21100.010.80221542-43
ATGCCGT18600.09.85730546-47
GCCGTCT20550.09.38541748-49
TCGTATG20450.08.93966942-43
ATCTCGT28600.08.21851340-41
CGTCTTC26400.07.75666350-51
GTATGCC25550.07.444720744-45
TCTCGTA24400.07.10324940-41
TGCTTGA39600.07.015725658-59
CTTGAAA43550.06.32473260-61
TCACGAT54100.06.27598894-95
GTCACGA56100.06.13689294-95
CGATCAG43150.05.94273494-95
AGTCACG62100.05.849828294-95
ACGATCA46650.05.802251394-95
Produced by FastQC (version 0.11.5)