FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24177532
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG90350.020.38977848-49
CGTATGC98300.018.83760846-47
CTCGTAT110300.016.48770344-45
TGCCGTC118850.015.60047950-51
CCGTCTT125950.014.89343652-53
ATCTCGT136350.013.26936442-43
TCGTATG103800.09.3440944-45
ATGCCGT106000.09.31841248-49
GCCGTCT113700.08.64548350-51
TGCTTGA224250.08.6410260-61
GGCCTTA228500.08.33372936-37
CCTTATC236150.08.05368938-39
TCTCGTA118900.08.02776242-43
TATCTCG123500.07.767203340-41
TTATCTC257800.07.53391440-41
GTATGCC137100.07.499735446-47
CTTGAAA264650.07.447635762-63
ACGTGGC265700.07.229931432-33
TCACGTG285100.06.61322930-31
CGTCTTC156850.06.524672552-53
Produced by FastQC (version 0.11.5)