FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24177532
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA66150.014.06863746-47
CGTATCA65050.013.40004150-51
CCGTATC76050.012.58237248-49
GCCGTAT69350.012.29149348-49
GTCGCCG87200.011.16058644-45
TCGCCGT75350.010.68100946-47
TGGTCGC101250.08.81549542-43
GGTCGCC102300.08.63151444-45
GTATCAT132650.07.520200350-51
GTGGTCG119700.07.179072442-43
ATCATTA190050.06.237216552-53
TCGGTGG170450.06.168512338-39
GATCGGG38550.05.76936671
ATCGGGA39200.05.55298472
TCTCGGT181800.05.456670336-37
CGGTGGT161450.05.410406640-41
GATCTCG212050.05.370792434-35
ATCTCGG209650.05.33743994-95
CATTAAA257300.05.26348154-55
GTGTAGA323500.05.08204794-95
Produced by FastQC (version 0.11.5)