FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20457550
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG951100.012.04154120
CGTCTGA849700.011.917339120
CACGTCT1061500.011.835295120
ATCACGA111350.011.266418120
GTCTGAA876700.011.201138120
GCACACG1442100.010.988499120
TCTGAAC795000.010.879945120
ACACGTC1139750.010.843677120
CACACGT1290100.010.766382120
GAACTCC626200.010.649539120
CTGAACT760000.010.543808120
TGAACTC689450.010.447401120
GAGCACA1853600.010.265333120
AGCACAC1668350.09.908469120
CATCACG146650.09.864267120
AGAGCAC2009150.09.829091120
AACTCCA599800.09.537098120
AAGAGCA2256700.09.32275120
TCCAGTC448550.09.206772120
GTCACAT273250.09.094315120
Produced by FastQC (version 0.11.5)