FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090019
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT52000.013.537429120
ACACGTC56450.012.68343120
CGTCTGA41400.011.771678120
CACACGT67950.010.891039120
AGCACAC95950.010.848127120
GCACACG74600.010.242797120
ACGTCTG46650.010.064053118-119
AGATCGG193200.09.747447120
TCGGAAG157050.09.73082120
AGAGCAC114800.09.695797120
CGGAAGA146950.09.584648118-119
TCTGAAC53000.09.535799120
AAGAGCA140000.09.497688120
GAGCACA108500.09.094269120
GATCGGA176000.09.024953120
ATCGGAA164350.08.936142118-119
GAAGAGC149300.08.825473120
AAGATCG60150.08.605828118-119
GTCTGAA57050.08.542461120
CAGATCG44550.08.508373120
Produced by FastQC (version 0.11.5)