FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090019
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA46950.014.069563120
AGCGTCG72950.013.664895120
CGTCGTG58350.012.349914120
GTCGTGT53700.012.077388120
GTAGGGA41300.011.632242120
GAGCGTC83750.011.615923120
AAGAGCG107750.011.592299120
GCGTCGG5809.255815E-611.38907120
GCGTCGT63250.011.297572118-119
GTGTAGG44950.011.222073120
CGTGTAG43200.011.189504118-119
AGAGCGT93750.011.14555120
TGTAGGG46400.09.965435120
AGATCGG189650.09.942608120
CGGAAGA145600.09.527394120
TCGGAAG154300.09.496147120
AAGATCG55550.09.296895120
GATCGGA173350.09.145419120
CAGATCG44450.09.051625120
ATCGGAA161000.08.839367118-119
Produced by FastQC (version 0.11.5)