FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127320169
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2069150.011.983076120
ACGTCTG1713550.011.706838120
CGTCTGA1533150.011.69097120
CACGTCT1964200.011.4318905120
CACACGT2476650.011.036306120
GCACACG2727250.010.935319120
ATCGGAA5861700.09.977065120
GATCGGA6292450.09.92348120
TCGGAAG5537450.09.901336120
CGGAAGA5220400.09.899203120
AGATCGG6901050.09.627441120
AGCACAC3592400.09.282302120
AGAGCAC4237700.09.054041120
GAGCACA4050400.08.908269120
TAGATCG1349100.08.6289425120
CAGATCG2457900.08.478921120
GAAGAGC5462950.08.385423120
AAGATCG1860700.08.191385120
AAGAGCA5214450.08.140603120
GAGATCG2577850.08.0308485120
Produced by FastQC (version 0.11.5)