FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32355561
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC539300.013.181997120
ACGTCTG443200.012.547964120
CGTCTGA384750.012.489561120
CACGTCT509250.012.110313120
GCACACG744700.011.809891120
CACACGT654500.011.631755120
GATCGGA1874750.011.132799120
TCGGAAG1613550.011.098268120
ATCGGAA1717750.010.893031120
CGGAAGA1517350.010.714622120
AGATCGG2069800.010.480774120
GAGCACA1107850.010.424208120
AGAGCAC1172550.010.063899120
AGCACAC961850.010.016857120
TAGATCG609600.09.752636120
AAGAGCA1431750.09.633078120
AAGATCG691400.09.292946120
CAGATCG527100.09.2304125120
GAAGAGC1539850.09.030843120
GAGATCG654950.08.912468120
Produced by FastQC (version 0.11.5)