FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9440093
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC324150.013.489345120
ACGTCTG259050.013.197771120
CGTCTGA231800.012.730956120
CACGTCT297800.012.688936120
CACACGT376300.011.715544120
GCACACG423300.011.619164120
TCTGAAC234450.011.333468120
AGCACAC505150.010.852634120
GAGCACA569150.010.675593120
AGAGCAC612900.010.412651120
GTCTGAA258400.010.283017120
ACGATCG4700.002490216210.20943120
GAACTCC189350.010.0099325120
ATCGGAA946350.09.849373120
AAGAGCA712700.09.829817120
CGGAAGA822250.09.811328120
TCGGAAG884450.09.779156120
TAGATCG322350.09.60133120
GAAGAGC776400.09.571833120
CTGAACT225550.09.493683120
Produced by FastQC (version 0.11.5)