FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67002638
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG2443800.010.832232120
CGTCTGA2211100.010.6561575120
ACACGTC2912950.010.6283245120
CACGTCT2706700.010.471717120
CACACGT3284500.010.242581120
GCACACG3598150.010.183495120
AGCACAC4218450.09.384422120
GTCTGAA2427550.09.0238495120
GAGCACA4637850.08.939424120
TCTGAAC2252000.08.935992120
AGAGCAC4955700.08.818873120
CGGAAGA6392250.08.520883120
GAAGAGC6035800.08.471394120
AAGAGCA5685450.08.407718120
TCGGAAG6789900.08.400948120
TGAACTC2049250.08.318106120
ATCGGAA7203900.08.305442120
GATCGGA7679150.08.123498120
AGATCGG8256350.07.9872494120
CTGAACT2204550.07.944422120
Produced by FastQC (version 0.11.5)