FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67002638
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG127000.016.300154120
CGTGTAG2354550.011.016754120
TCGTGTA2544050.010.85183120
CGTCGTG3064800.010.723016120
GTCGTGT2810700.010.708272120
AGCGTCG3690950.010.386544120
GCGTCGT3331500.010.174384120
GTGTAGG2310200.010.137371120
GAGCGTC4103000.09.8553095120
GTAGGGA2007100.09.695145120
AAGAGCG4920450.09.471632120
TGTAGGG2253200.09.43779120
AGAGCGT4444500.09.368074120
TAGGGAA1964150.08.749328120
CGGAAGA6322450.08.480747120
TCGGAAG6708900.08.4412155120
GAAGAGC5966550.08.427467120
ATCGGAA7116750.08.317479120
GATCGGA7560600.08.102206120
AGATCGG8127850.07.990029120
Produced by FastQC (version 0.11.5)