FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88023281
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2884350.3276803553823448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG159800.022.442547120
CGTGTAG1539350.012.850764120
TCGTGTA1674950.012.811034120
CGTCGTG2087300.012.605608120
GTCGTGT1895350.012.123181120
AGCGTCG2600050.011.926441120
GAGCGTC2978100.011.459353120
GTGTAGG1605650.011.063054120
GCGTCGT2283350.011.060247120
AAGAGCG3747750.010.920496120
AGAGCGT3266400.010.7771225120
GTAGGGA1414800.010.173407120
TGTAGGG1654350.010.0946665120
GATCGGA6215450.010.08833120
CGGAAGA5026650.010.054184120
ATCGGAA5736950.010.053331120
TCGGAAG5378850.09.947561120
AGATCGG6806100.09.652388120
TAGATCG2110200.09.143795120
GAAGAGC4895950.09.014625120
Produced by FastQC (version 0.11.5)