FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17879269
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG38650.027.664843120
CGTGTAG654550.012.976701120
GTGTAGG629800.012.847618120
TCGTGTA720100.012.813155120
GTAGGGA518650.012.693864120
CGTCGTG895400.012.230023120
GTCGTGT807100.012.094383120
TGTAGGG597450.012.055224120
AGCGTCG1107050.011.573942120
TAGGGAA490850.011.540393120
GAGCGTC1247250.011.448098120
GCGTCGT983350.011.215592120
GAGTGTA186800.011.190772120
AAGAGCG1532350.010.537292120
AGAGCGT1366700.010.460717120
AGTGTAG180950.010.191485120
CGGAAGA1997650.09.381945120
AGAGTGT290100.09.110929120
AAGAGTG342950.09.108151120
GAAGAGC1837700.09.008704120
Produced by FastQC (version 0.11.5)