FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56685345
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1829210.32269539860787655No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG95700.020.4617120
CGTGTAG1854900.09.297128120
AGCGTCG2728050.09.278511120
TCGTGTA1984100.09.25482120
CGTCGTG2311750.09.205902120
GAGCGTC3011400.09.101607120
GTGTAGG1861250.08.926548120
GTCGTGT2157550.08.878298120
AAGAGCG3579300.08.724847120
GCGTCGT2477600.08.541174120
AGAGCGT3241950.08.4562025120
CGGAAGA4535250.08.17979120
GTAGGGA1643650.08.16413120
TGTAGGG1823500.08.070425120
TCGGAAG4805200.08.04027120
ATCGGAA5070300.08.03334120
GATCGGA5386350.07.887605120
AGATCGG5783000.07.8316674120
TAGGGAA1642750.07.80661120
GAAGAGC4342700.07.532756120
Produced by FastQC (version 0.11.5)