FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44952202
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT335950.012.304216120
AGTCACG361450.012.303045120
CGTCTGA1505300.012.2490635120
CACGTCT1885600.012.162524120
ACACGTC2056550.011.910385120
ACGTCTG1684600.011.839564120
GCACACG2597850.011.562602120
CACACGT2343350.011.283243120
TCTGAAC1483550.011.031436120
GTCTGAA1638550.011.006558120
AGCACAC3062800.010.527392120
GAGCACA3401500.010.421563120
TCACGTG344950.010.32371120
AGAGCAC3681300.010.298928120
CTGAACT1438050.09.968389120
TGAACTC1313050.09.889413120
GAACTCC1170900.09.818905120
CGGAAGA4952700.09.711229120
AAGAGCA4237600.09.610966120
TCGGAAG5328050.09.47268120
Produced by FastQC (version 0.11.5)