FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46159621
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT446150.011.064184120
ACACGTC447850.011.048972120
CGTCTGA342700.010.95622120
ACGTCTG381750.010.809607120
ATCGGAA1270800.010.6809120
GATCGGA1366100.010.64706120
TCGGAAG1232100.010.4809120
AGATCGG1512850.010.28826120
TAGATCG270400.010.114831120
CACACGT558000.09.88904120
CGGAAGA1184400.09.87499120
GCACACG630900.09.487908120
CAGATCG638550.08.744908120
AAGATCG466750.08.609758120
GAGATCG562500.08.359764120
AGCACAC878350.08.105571120
AGAGCAC1034950.08.032382120
GAAGAGC1382100.07.776766120
GAGCACA1015250.07.721525120
AAGAGCA1321750.06.9928436120
Produced by FastQC (version 0.11.5)