FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58396456
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG121000.012.993382120
CGTGTAG2136900.09.348371120
AGCGTCG3164400.09.312899120
CGTCGTG2680900.09.264482120
GTCGTGT2494950.09.218988120
TCGTGTA2287750.09.175242120
AAGAGCG4138650.09.060625120
GCGTCGT2882600.09.059637120
GAGCGTC3488400.08.941622120
GTGTAGG2121400.08.839624120
AGAGCGT3752150.08.656936120
TCGGAAG5574700.08.418739120
CGGAAGA5255250.08.364126120
TGTAGGG2104350.08.261081120
ATCGGAA5918850.08.181681120
GTAGGGA1871850.08.168357120
GATCGGA6266700.07.953519120
GAAGAGC4994150.07.9422984120
AGATCGG6731100.07.862119120
TAGGGAA1850000.07.687461120
Produced by FastQC (version 0.11.5)