FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56355275
Sequences flagged as poor quality0
Sequence length126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG748750.011.885458120
AGCGTCG915150.011.527581120
TCGTGTA639050.011.127437120
GCGTCGT813250.010.913291120
GTCGTGT707350.010.892869120
GAGCGTC1059600.010.777262120
AGAGCGT1164350.010.570456120
AAGAGCG1327550.010.568303120
CGTGTAG588850.010.547445120
GATCGGA2140350.010.023119120
ATCGGAA1998500.09.803715120
CGGAAGA1797200.09.739836120
TCGGAAG1890400.09.719929120
AGATCGG2343900.09.641684120
GTGTAGG693350.09.24337120
CAGATCG1156300.09.040433120
AAGATCG605350.08.366571120
ACGACGC43700.08.23811418-19
GAAGAGC1931750.07.980402120
TAGATCG235900.07.9112306120
Produced by FastQC (version 0.11.5)