FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103018222
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG256400.015.493551120
CGTGTAG4143000.010.382312120
TCGTGTA4449100.010.040265120
GTCGTGT4899150.09.931252120
CGTCGTG5310700.09.91983120
GTGTAGG4049100.09.913196120
GTAGGGA3493050.09.706321120
AGCGTCG6319450.09.588862120
TGTAGGG3917400.09.477486120
TAGGGAA3360900.09.343426120
GAGCGTC6993050.09.290787120
GCGTCGT5735550.09.278154120
AGAGCGT7542950.08.875202120
AAGAGCG8293650.08.712199120
GAGTGTA1655200.08.070226120
CGGAAGA10432450.07.9597235120
TCGGAAG11027800.07.9506392120
GAAGAGC9861400.07.8474293120
AGTGTAG1612600.07.7810526120
ATCGGAA11602200.07.694599120
Produced by FastQC (version 0.11.5)