FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77065481
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG111750.011.223191120
CGTCGTG1740300.011.148058120
CGTGTAG1358850.010.824041120
TCGTGTA1470850.010.558774120
AGCGTCG2098250.010.341626120
GTCGTGT1616600.010.293544120
GAGCGTC2369400.010.216805120
GCGTCGT1884400.09.938899120
AGAGCGT2580500.09.790297120
AAGAGCG2878700.09.582872120
TCGGAAG4025050.09.401568120
CGGAAGA3785800.09.13183120
ATCGGAA4261950.08.997256120
GATCGGA4541600.08.9678135120
GTGTAGG1445300.08.889479120
AGATCGG4930200.08.809914120
GTAGGGA1300200.08.376915120
TAGATCG688000.08.303595120
GAGATCG1787700.08.146908120
CAGATCG1915450.08.1204815120
Produced by FastQC (version 0.11.5)