FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50737143
Sequences flagged as poor quality0
Sequence length126
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG123000.015.706215120
GTCGTGT711350.012.617376120
CGTCGTG778150.012.497999120
CGTGTAG582000.012.401193120
AGCGTCG972450.012.400795120
GCGTCGT843000.011.3977995118-119
TCGTGTA610150.011.327567120
GAGCGTC1132100.011.261465120
AAGAGCG1430850.010.767669120
AGAGCGT1234750.010.364124120
ATCGGAA2142500.010.05297120
TCGGAAG2039800.010.032632120
GTGTAGG639900.09.8727255120
GATCGGA2305200.09.692189120
AGATCGG2549250.09.578616120
GATCGGG106200.09.49086120
TAGATCG414850.09.313557120
CGGAAGA1924050.08.958599120
TGTAGGG701700.08.575713120
CAGATCG1008250.08.408038120
Produced by FastQC (version 0.11.5)