FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8781490
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG295300.011.987368120
CGTCTGA261450.011.818268120
ACACGTC352700.011.482424120
CACACGT405450.011.068775120
CACGTCT328350.010.598056120
GTCTGAA280550.010.393486120
GCACACG455550.010.3256120
TCTGAAC254250.010.052727120
AGCACAC534750.010.041706120
CGGAAGA841850.010.006165120
AGAGCAC632850.09.954598120
CTGAACT250900.09.804342120
GAGCACA591850.09.752112120
ATCGGAA961550.09.659048120
TCGGAAG896050.09.508048120
GATCGGA1038150.09.385582120
AAGAGCA723950.09.083166120
TAGATCG325800.09.0788555120
AGATCGG1127500.08.8919115120
GAAGAGC782150.08.852195120
Produced by FastQC (version 0.11.5)