FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78224130
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA1184700.012.63759120
CGTCGTG1440900.012.282781120
CGTGTAG1088100.012.032006120
AGCGTCG1785200.011.9760475120
GTCGTGT1327150.011.941799120
GCGTCGT1580350.011.426955120
GAGCGTC2062650.011.395823120
GCGTCGG125500.010.814715120
AGAGCGT2255250.010.61434120
GTGTAGG1177500.010.59827120
AAGAGCG2565100.010.5355835120
ATCGGAA3865750.09.740565120
GATCGGA4156600.09.661476120
TCGGAAG3661100.09.642028120
CGGAAGA3459300.09.5934105120
GTAGGGA1049850.09.524394120
AGATCGG4529850.09.337363120
TGTAGGG1218800.09.0959835120
CAGATCG1587150.08.623364120
AAGATCG1561150.08.32844120
Produced by FastQC (version 0.11.5)