FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16015365
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT435500.012.411001120
CGTCTGA268000.012.329803120
CACGTCT343700.011.873227120
ACGTCTG305000.011.722095120
ACACGTC364850.011.66336120
GCACACG479100.011.419743120
GAGCACA676050.010.372077120
TCGGAAG1014600.010.351879120
AGCACAC600350.010.346508120
CGGAAGA947950.010.203498120
ATCGGAA1084950.010.196577120
AGAGCAC728350.09.949586120
GATCGGA1170550.09.805717120
AGATCGG1284150.09.767886120
TCTGAAC308500.09.579547120
GTCTGAA341200.09.543482120
TAGATCG366250.09.53167120
AAGAGCA868950.09.150117120
GAAGAGC917900.09.121166120
AAGATCG447100.09.073479120
Produced by FastQC (version 0.11.5)