FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16015365
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG26750.015.4914875120
CGTCGTG382800.013.884785120
CGTGTAG285800.012.692365120
AGCGTCG479100.012.39761120
TCGTGTA306200.012.3174925120
GAGCGTC557100.012.074042120
GCGTCGT423300.011.747621120
GTCGTGT348000.011.511036120
GTGTAGG293500.011.233939120
AAGAGCG700250.011.209605120
AGAGCGT613250.011.115426120
GTAGGGA254350.010.71993120
TCGGAAG1011550.010.295075120
TGTAGGG301500.010.1988325120
ATCGGAA1075500.010.179913120
CGGAAGA945300.010.133485120
GATCGGA1159100.09.766934120
AGATCGG1266900.09.708574120
TAGATCG358700.09.510094120
TAGGGAA253400.09.314377120
Produced by FastQC (version 0.11.5)