FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67409884
Sequences flagged as poor quality0
Sequence length126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC579200.011.258277120
CACGTCT570600.010.954862120
ACGTCTG494750.010.864085120
GCACACG773350.010.821042120
GATCGGA1810200.010.57809120
CGTCTGA439050.010.534437120
TCGGAAG1601800.010.497498120
ATCGGAA1697700.010.469879120
CGGAAGA1521100.010.332715120
AGATCGG1997200.010.131313120
CACACGT724800.010.022981120
TAGATCG493950.09.351443120
AAGATCG677950.08.81318120
AGAGCAC1347950.08.602586120
AGCACAC1167550.08.277344120
CAGATCG696050.08.124042118-119
GAGCACA1308550.08.050172120
GAAGAGC1779550.07.911773120
GAGATCG677750.07.2279954118-119
AAGAGCA1823600.07.151562120
Produced by FastQC (version 0.11.5)