FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17092610
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA469350.012.525535120
AGTCACG125800.012.112118120
CACGTCT595850.012.081234120
ACACGTC644950.011.868385120
ACGTCTG531550.011.849833120
CACACGT745450.011.290318120
ACGGCTA71200.011.205692120
GCACACG816750.011.024492120
TCTGAAC479650.010.718238120
GTCACGG117000.010.510782120
GAGCACA1079650.010.4958105120
GTCTGAA527750.010.173299120
AGCACAC978800.010.130851120
AGAGCAC1171250.010.1103115120
CGGAAGA1573150.09.864914120
TCACGGC113650.09.817718120
TCGGAAG1693650.09.549117120
GAAGAGC1448800.09.423903120
ATCGGAA1815700.09.290482120
GATCGGA1956850.09.257984120
Produced by FastQC (version 0.11.5)