FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31843289
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG525150.011.504698120
ACACGTC631050.011.447002120
CACACGT735350.011.202256120
CACGTCT591550.010.700154120
CGTCTGA471750.010.695798120
GCACACG817650.09.94996120
TCGGAAG1591050.09.32544120
GATCGGA1789050.09.312847120
CGGAAGA1500550.09.132185120
ATCGGAA1671700.09.105236120
AGAGCAC1202700.09.094073120
AGATCGG1940550.08.870228120
AGCACAC1033350.08.784571120
GAGCACA1139050.08.712078120
AGTCACG159700.08.565624120
AAGATCG545800.08.497184120
AAGAGCA1462200.08.38283120
GAAGAGC1520300.08.236112120
GTCTGAA634500.08.198163120
CAGATCG796950.08.130584120
Produced by FastQC (version 0.11.5)