FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65854127
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA1038300.012.53544120
ACACGTC1518100.012.448709120
ACGTCTG1161750.012.09305120
CACGTCT1324550.011.663737120
CACACGT1781950.011.354072120
GCACACG2014300.011.300296120
AGCACAC2452600.09.873755120
AGAGCAC2952600.09.752505120
TCGGAAG4032650.09.7183895120
CGGAAGA3796850.09.67781120
GATCGGA4590300.09.498613120
ATCGGAA4262100.09.491273120
GAGCACA2769100.09.478683120
AGATCGG4994700.09.019493120
GTCTGAA1298650.09.004399120
ACGTCGA134100.08.917196120
GAAGAGC3745050.08.91474120
AAGAGCA3467850.08.901298120
CAGATCG1990700.08.560605120
TCTGAAC1210500.08.473694120
Produced by FastQC (version 0.11.5)