FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65854127
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG154900.012.8309765120
TCGTGTA1264550.012.735204120
CGTGTAG1176500.012.545071120
GTCGTGT1439150.012.446015120
CGTCGTG1589900.012.429144120
AGCGTCG1966350.011.921535120
GTGTAGG1200800.011.66114120
GCGTCGT1736600.011.572817120
GAGCGTC2269350.011.173847120
AAGAGCG2802750.010.737665120
AGAGCGT2476300.010.724989120
TGTAGGG1219050.010.225604120
GTAGGGA1044750.010.172883120
TAGGGAA1030850.010.071233120
TCGGAAG3995400.09.675509120
CGGAAGA3776100.09.483689120
GATCGGA4541700.09.472855120
ATCGGAA4213050.09.466413120
AGATCGG4943100.09.066828120
GAAGAGC3726600.08.781464120
Produced by FastQC (version 0.11.5)