FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12737862
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG71900.015.45963120
AGTCACG130700.012.917754120
GTCACGT116600.012.161013120
CGTCTGA525500.011.708022120
TCACGTC110850.011.599369120
ACGTCTG587350.011.590155120
CGTCCGC74800.011.486579120
GTCCGCA70100.011.228185120
ACACGTC702300.011.113293120
GTCTGAA547200.011.11196120
CACGTCT651150.011.045094120
CACACGT793550.011.008962120
TCTGAAC493650.010.893317120
GCACACG883950.010.732742120
CTGAACT471450.010.590628120
GAACTCC391450.010.437317120
TGAACTC429850.010.203806120
AACTCCA371750.09.923701120
AGCACAC1016750.09.774119120
TCCGCAC69200.09.637695120
Produced by FastQC (version 0.11.5)