FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21294479
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG471300.09.941558120
AGCGTCG561050.09.891019120
GAGCGTC639800.09.798801120
GCGTCGT506900.09.764107120
GTCGTGT436850.09.57197120
TCGTGTA397600.09.415407120
AGAGCGT688500.09.410673120
AAGAGCG765500.09.208598120
CGTGTAG378700.09.172423120
GATCGGA1170650.09.024708120
TCGGAAG1049650.08.887647120
ATCGGAA1091800.08.7918120
AGATCGG1272950.08.747169120
TAGATCG152650.08.724832120
CGGAAGA1006200.08.669239120
CAGATCG572600.08.214206120
GAGATCG448550.08.078429120
GTAGGGA371500.07.848349120
GTGTAGG410200.07.8099174120
AAGATCG336850.07.5158267120
Produced by FastQC (version 0.11.5)