FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60146920
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1139700.010.77066120
CGTCTGA870300.010.492353120
ACGTCTG982300.010.322141120
CACGTCT1095800.010.024998120
CACACGT1357850.09.884198120
CGGAAGA2641550.09.632448120
GCACACG1479000.09.516716120
ATCGGAA3014050.09.483061120
GATCGGA3237300.09.464772120
TCGGAAG2819900.09.423217120
AGATCGG3524100.09.34655120
AGCACAC1832450.08.918714120
GTCACGA273600.08.881087120
AGAGCAC2101700.08.8694725120
GAGCACA1981050.08.755479120
TAGATCG1090150.08.624024120
GAAGAGC2592400.08.574592120
AGTCACG310650.08.381961120
AAGAGCA2581100.08.147239120
CAGATCG822700.08.029214120
Produced by FastQC (version 0.11.5)