FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60146920
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG79800.011.35289120
CGTCGTG1174800.011.153754120
AGCGTCG1426100.011.15301120
GTCGTGT1089400.010.981715120
CGTGTAG919000.010.967967120
TCGTGTA984400.010.915819120
GAGCGTC1613250.010.443061120
GCGTCGT1277150.010.222317120
AAGAGCG2044250.010.046279120
AGAGCGT1769350.09.982893120
CGGAAGA2624650.09.651153120
ATCGGAA2979550.09.534582120
TCGGAAG2795700.09.481292120
GATCGGA3199500.09.426688120
AGATCGG3494450.09.331535120
GTGTAGG976400.09.278581120
TAGATCG1070750.08.926065120
GAAGAGC2581450.08.51345120
GTAGGGA894050.08.240791120
TGTAGGG1007750.08.150476120
Produced by FastQC (version 0.11.5)