FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69944534
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG2599950.010.549139120
TCGTGTA2812950.010.336817120
CGTCGTG3356450.010.33055120
GTCGTGT3089200.010.185334120
GTGTAGG2547100.09.983738120
AGCGTCG3992200.09.825956120
GCGTCGT3635500.09.72737120
GTAGGGA2185350.09.574834120
GAGCGTC4408100.09.377921120
TGTAGGG2469950.09.1637745120
AGAGCGT4764150.09.153032120
AAGAGCG5239600.08.991108120
TAGGGAA2136000.08.911373120
GAGTGTA1047450.08.315897120
CGGAAGA6676950.08.216395120
TCGGAAG7078600.08.201892120
GAAGAGC6343650.08.077856120
GATCGGA7961950.08.011469120
ATCGGAA7486700.07.95673120
GTAGATC831200.07.9245133120
Produced by FastQC (version 0.11.5)