FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93732773
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1515300.012.325864120
CGTCTGA1106550.012.213873120
CACGTCT1434200.012.147124120
ACGTCTG1249100.011.969835120
GCACACG1999650.011.507113120
CACACGT1797900.011.140502120
CGGAAGA3894600.010.069762120
ATCGGAA4399200.010.045809120
TCGGAAG4153550.010.02504120
GATCGGA4735750.09.882622120
AGCACAC2605950.09.683108120
GAGCACA2957450.09.428348120
AGATCGG5164950.09.395317120
AGAGCAC3116750.09.305086120
AAGATCG1680900.08.962878120
GAAGAGC4017000.08.655888120
CAGATCG1908100.08.557038120
TAGATCG805100.08.471904120
AAGAGCA3847200.08.438103120
GAGATCG1795600.08.132642120
Produced by FastQC (version 0.11.5)