FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7672632
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC45600.013.704954120
ACGTCTG231800.012.521109120
CGTCTGA206550.012.277116120
ACACGTC280300.012.133956120
AGTCACG51600.011.878444120
GCACACG358400.011.283819120
CACGTCT258900.011.256899120
CACACGT321400.011.087098120
TCTGAAC212600.010.4014435120
GTCTGAA233250.010.150412120
GAGCACA472750.010.067061120
AGCACAC426950.09.992874120
ACGCCAA37300.09.827206120
CGGAAGA665000.09.686841120
AGAGCAC508900.09.48183120
CTGAACT208300.09.289144120
ATCGGAA756300.09.065684120
AAGAGCA591600.09.050209120
TCGGAAG709900.09.048771120
AACTCCA171450.08.867314120
Produced by FastQC (version 0.11.5)