FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7672632
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG16600.013.022422120
CGTGTAG222250.012.779583120
GTCGTGT272100.012.005153120
GTGTAGG216650.011.890384120
GTAGGGA180550.011.873207120
CGTCGTG298650.011.782363120
TCGTGTA241900.011.691826120
AGCGTCG366400.011.291745120
GCGTCGT326500.011.237128120
GAGCGTC415750.010.991317120
TGTAGGG213000.010.853716120
TAGGGAA174000.010.732687120
AAGAGCG507100.010.242827120
AGAGCGT451000.09.999096120
CGGAAGA659800.09.637868120
GAGTGTA73350.09.496317120
GTAGATC93500.09.440675120
TAGATCG278800.09.196708120
ATCGGAA746700.08.998731120
TCGGAAG701250.08.973993120
Produced by FastQC (version 0.11.5)