FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103294220
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG641150.011.200021120
ACGTCTG4462600.010.977558120
AGTCACG1225300.010.878924120
CGTCTGA4048550.010.814056120
GTCACGT1136850.010.764934120
ACACGTC5319850.010.424252120
CACGTCT4927750.010.423436120
CACACGT5976750.010.177876120
GTCTGAA4368800.09.627248120
GCACACG6513700.09.582938120
GAACTCC3105600.09.534852120
TCTGAAC4029250.09.491619120
TCACGTT1144400.09.283784120
CTGAACT3871450.09.226129120
TGAACTC3494750.09.182075120
AGCACAC7509950.08.931573120
CACGTTT1115650.08.614278120
GAGCACA8272750.08.542397120
AGAGCAC8810850.08.431258120
ACGTTTC933550.08.3410635120
Produced by FastQC (version 0.11.5)