FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103294220
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG211600.016.616856120
CGTGTAG4415100.011.1100025120
TCGTGTA4791400.010.670753120
CGTCGTG5747500.010.555578120
GTCGTGT5272100.010.430756120
GTGTAGG4264950.010.33625120
GTAGGGA3641500.010.321394120
TAGGGAA3508650.010.163251120
TGTAGGG4104400.010.133881120
AGCGTCG6845000.09.737969120
GCGTCGT6219700.09.465749120
GAGTGTA1576950.09.390617120
GAGCGTC7537100.09.263313120
AGTGTAG1518050.09.118606120
AAGAGCG8901400.08.765679120
AGAGCGT8129100.08.762911120
GTGTAGA1533050.08.434471120
AGAGTGT2100450.08.204252120
GTAGATC1383100.08.077799120
AAGAGTG2426900.08.057483120
Produced by FastQC (version 0.11.5)