FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29808894
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA769450.011.346602120
ACGTCTG862350.011.341938120
ACACGTC1037900.011.082809120
CACGTCT961600.010.857705120
CACACGT1195750.010.783984120
GCACACG1339450.010.330375120
GTCTGAA863000.09.880218120
TCTGAAC788100.09.532488120
AGCACAC1574900.09.4984455120
AGAGCAC1851150.09.361358120
GAGCACA1737650.09.289091120
CGGAAGA2404300.09.179232120
CTGAACT779150.09.102899120
TCGGAAG2560000.09.033479120
ATCGGAA2717850.08.952589120
GATCGGA2914250.08.758988120
GAAGAGC2296000.08.6687565120
AGATCGG3149400.08.472714120
AAGAGCA2135850.08.3579445120
TGAACTC731000.08.249582120
Produced by FastQC (version 0.11.5)