FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19917122
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG688950.010.451749120
TCGTGTA564400.010.089616120
AGCGTCG825100.09.985261120
CGTGTAG535750.09.878746120
GCGTCGG69250.09.704958120
GTCGTGT626250.09.533904120
AAGAGCG1104150.09.3583765120
GAGCGTC925950.09.254141120
GCGTCGT740250.09.249167120
GTGTAGG544100.09.241889120
AGAGCGT990850.08.9265785120
CGGAAGA1421850.08.824614120
TCGGAAG1493350.08.775795120
ATCGGAA1566150.08.440663120
AGATCGG1812650.08.408435120
GATCGGA1675850.08.249788120
GTAGGGA473050.08.245211120
TGTAGGG546300.08.172166120
TAGATCG487650.08.146015120
GAAGAGC1398600.08.010253120
Produced by FastQC (version 0.11.5)