FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90800662
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG2624450.010.3118725120
ACACGTC3095900.010.286012120
CACGTCT2896450.010.039452120
CGTCTGA2400550.09.991599120
CACACGT3490500.09.791779120
GCACACG3787650.09.747441120
GAGCACA4959650.08.901655120
CGGAAGA6797900.08.869401120
TCGGAAG7235400.08.775872120
AGCACAC4483250.08.773018120
AGAGCAC5286850.08.718401120
CGTACGT469650.08.596936120
ATCGGAA7678100.08.522255120
GATCGGA8203400.08.44239120
GAAGAGC6462050.08.33977120
CCGTACG507100.08.328793120
AGATCGG8831600.08.184923120
GTCTGAA2782950.08.181056120
AAGAGCA6155650.08.16135120
TAGATCG1280400.07.979432120
Produced by FastQC (version 0.11.5)