FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90800662
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG166000.012.143275120
CGTGTAG2572050.010.596635120
TCGTGTA2773600.010.4149475120
GTCGTGT3055500.010.365111120
CGTCGTG3314000.010.338662120
AGCGTCG3954200.09.940767120
GAGCGTC4417300.09.934868120
GCGTCGT3586650.09.79695120
AAGAGCG5295950.09.436388120
AGAGCGT4783400.09.412796120
GTGTAGG2577050.09.35155120
TGTAGGG2560950.08.932445120
CGGAAGA6822200.08.822893120
TCGGAAG7247750.08.753502120
GTAGGGA2266600.08.713439120
ATCGGAA7675250.08.5473385120
GATCGGA8194800.08.436641120
TAGGGAA2285500.08.297512120
GAAGAGC6552750.08.267397120
AGATCGG8828550.08.1735115120
Produced by FastQC (version 0.11.5)