FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72350107
Sequences flagged as poor quality0
Sequence length126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG93000.019.238127120
TCGTGTA2281850.010.819208120
CGTCGTG2712750.010.807032120
CGTGTAG2086300.010.7915535120
GTCGTGT2499850.010.341643120
GTGTAGG2094650.010.152349120
AGCGTCG3248850.010.097422120
GCGTCGT2947500.09.822065120
GAGCGTC3605650.09.656039120
AGAGCGT3916800.09.532759120
GTAGGGA1825100.09.391802120
TGTAGGG2051050.09.390474120
AAGAGCG4327000.09.339468120
TAGGGAA1857300.08.67944120
CGGAAGA5556050.08.620994120
TCGGAAG5906500.08.615693120
ATCGGAA6274400.08.3238945120
GATCGGA6684900.08.212413120
GAAGAGC5276450.08.117471120
AGATCGG7180900.07.8642187120
Produced by FastQC (version 0.11.5)